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10ml 50ml min medium low pressure protein purification system
10ml 50ml min medium low pressure protein purification system
Product details
PPS-2 low-pressure protein purification system: 1-5Mp (Application level: Small experimental type, semi prepared type,)
System Characteristics
Modular design
Convenient for users to choose and match the required equipment, and with a small footprint. All valve and other pipeline joints are located on the right side, and a reasonable pipeline layout greatly reduces dead volume in the pipeline. Connecting the instrument backend to a fixed column can form a whole, making it convenient for users to choose and match.
infusion system
Dual plunger parallel mode, floating plunger design.
system detection
The 280nm254nm and 190nm-700nm UV detectors (or other online detectors) use imported deuterium and tungsten lamp light sources, ensuring reliable quality.
PH conductivity
The spectral lines of online pH conductivity detection facilitate users to grasp experimental data and conditions in real time, greatly shortening the experimental process.
Component collection
XY axis mode, high-precision positioning, powerful automatic collection capability, supports full collection, and peak collection (threshold, slope, conductivity).
software
The automatic buffer preparation function facilitates quick exploration of purification processes, and the corresponding parameters are programmed according to on-site process requirements. Real time monitoring and control of flow paths; The same screen displays UV, gradient spectral lines, and collection tube numbers. Automatic software control improves the purity and repeatability of collection, enabling the system to obtain more separation information of samples and impurities in a timely manner, making the tedious chromatographic purification process easy and convenient.
technical specifications
| Light source s | Imported deuterium lamps, tungsten lamps, etc |
| Two wavelength range configurations | 1. Two wavelengths of 280nm254nm and 190nm-700nm can be continuously set online without changing the lamp, and two wavelengths can be detected simultaneously. |
| Wavelength online scanning | Full range scanning from 190nm to 700nm |
| Wavelength accuracy | ±1nm |
| Wavelength reproducibility: | 0.2nm、 |
| baseline noise | 0-5AU ±0.5×10-5AU |
| Range of absorbance | -5AU—5AULinear: ± 2%, between 0-2 AU |
| pump head | Material: Stainless steel pump head (ceramic and PEEK optional), imported ruby ball one-way valve, high precision, long service life. |
| flow rate range | 0.01-10ml/min、0.1-50ml/min、 |
| Flow velocity accuracy | ±1% |
| Traffic reproducibility | ±1% |
| Pressure Range | 1-5mp |
| Online quaternary gradient mixer | Curve: Straight Line, Ladder, Convex Line, Concave Line, Electric Hybrid Volume: 2ml5ml |
| Online conductivity meter, online pH meter | Conductivity measurement range: 1us-99.99ms; Equipped with temperature detector: temperature range: 0-99 ° C; temperature accuracy: ± 1.5 ° C between 4 ° C-45 ° C, accurately reflecting temperature changes. PH measurement range: 0-14.00pH |
| Sample pump | 0.1-10ml/min、 Single chip microcontroller with 32 subdivisions, small pulsation, and speed display. |
| Sample size and mode | Set the sample size according to the user's process requirements, （Sample can be loaded onto a quantitative tube, 200ul-2ml, or add a conversion valve to the sample pump) |
| Component collection | Peak collection: automatically collected based on volume, time, threshold, slope, conductivity. Collection mode: Normal mode, Z-type collection, making collection more intelligent and user-friendly. High precision three-way valve is used while bottle cutting technology is applied. The waste liquid has an independent channel, and the bottle switching process is leak free. The supporting software can collect wavelength signals in real time. Capacity: 100 tubes Collection tubes: 12ml |
| Biological freezer | Temperature range: 1-10 ℃, power: 850w, size: 1800 * 800 * 1960 (outer size) 1680 * 630 * 1400 (inner size) |
| power supply | 220VAC/110VAC |
| Power frequency | 50Hz |
| operation temperature | 1-40℃ |
| Control Interface | RS-232C(USB)， Using a PC software control platform based on Windows XP or Windows 7, it can display UV, pH, conductivity, gradient spectra, and display the collection tube number. |
Example of Purification Atlas for Protein Purification System
Purification Atlas of the School of Food Science, Lanzhou Agricultural University
Purification spectrum of Biochemistry Department, Beijing Institute of Electronic Technology
Purification profile of Tianjin International Medical Joint Research Institute
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